Important Publications


Cloning and sequencing of human Eppin: a novel family of protease inhibitors expressed in the epididymis and testis.

Richardson RT, Sivashanmugam P, Hall SH, Hamil KG, Moore PA, Ruben SM, et al.

Gene. 2001 May 30;270(1–2):93–102. PMID:11404006 


Antimicrobial activity of human EPPIN, an androgen-regulated, sperm-bound protein with a whey acidic protein motif.

Yenugu S, Richardson RT, Sivashanmugam P, Wang Z, O'Rand MG, French FS, Hall SH.

Biol Reprod. 2004 Nov;71(5):1484-90. Epub 2004 Jun 30. PMID:15229136

 DOI: 10.1095/biolreprod.104.031567 


Reversible immunocontraception in male monkeys immunized with eppin.

O'Rand MG, Widgren EE, Sivashanmugam P, Richardson RT, Hall SH, French FS, VandeVoort CA, Ramachandra SG, Ramesh V, Jagannadha Rao A.

Science. 2004 Nov 12;306(5699):1189-90. PMID:15539605 

DOI: 10.1126/science.1099743 


Association of eppin with semenogelin on human spermatozoa.

Wang Z, Widgren EE, Sivashanmugam P, O'Rand MG, Richardson RT.

Biol Reprod. 2005 May;72(5):1064-70. Epub 2004 Dec 8. PMID:15590901

DOI: 10.1095/biolreprod.104.036483 


Characterization of an eppin protein complex from human semen and spermatozoa.

Wang Z, Widgren EE, Richardson RT, O'Rand MG.

Biol Reprod. 2007 Sep;77(3):476-84. Epub 2007 Jun 13. PMID:17567961

DOI: 10.1095/biolreprod.107.060194 


Inhibition of human sperm motility by contraceptive anti-eppin antibodies from infertile male monkeys: effect on cyclic adenosine monophosphate.

O'Rand MG, Widgren EE, Beyler S, Richardson RT.

Biol Reprod. 2009 Feb;80(2):279-85. Epub 2008 Oct 22. PMID: 18945989

DOI: 10.1095/biolreprod.108.072942 


Analysis of recombinant human semenogelin as an inhibitor of human sperm motility.

Mitra A, Richardson RT, O'Rand MG.

Biol Reprod. 2010 Mar;82(3):489-96. Epub 2009 Nov 4.PMID: 19889947

DOI: 10.1095/biolreprod.109.081331 


Epididymal protein targets: a brief history of the development of epididymal protease inhibitor as a contraceptive.

O’Rand MG, Widgren EE, Hamil KG, Silva EJ, Richardson RT. J Androl. 2011 Dec;32(6):698–704. Epub 2011 Mar 25. Review. PMID: 21441428

DOI: 10.2164/jandrol.110.012781 


Loss of calcium in human spermatozoa via EPPIN, the semenogelin receptor.

O'Rand MG, Widgren EE Biol Reprod. 2012 Feb 29;86(2):55. doi: 10.1095/biolreprod.111.094227. Print 2012 Feb. PMID: 22075473 

DOI: 10.1095/biolreprod.112.101832 


Characterization of EPPIN's semenogelin I binding site: a contraceptive drug target.

Silva EJR, Hamil KG, Richardson RT, O’Rand MG.

Biol Reprod. 2012 Sep;87(3):56, Print 2012 Sep. PMID:22699487

DOI: 10.1095/biolreprod.112.101832 


Interacting proteins on human spermatozoa: adaptive evolution of the binding of semenogelin I to EPPIN.

Silva EJR, Hamil KG, O’Rand MG.. PloS One. 2013;8(12):e82014. eCollection 2013. PMID:24312623.

DOI: 10.1371/journal.pone.0082014 


Non-hormonal male contraception: A review and development of an Eppin based contraceptive.

O’Rand MG, Silva EJR, Hamil KG. 

Pharmacol Ther. 2016 Jan;157:105–11. PMID: 26593445

DOI: 10.1016/j.pharmthera.2015.11.004 

Hot off the presses


Inhibition of sperm motility in male macaques with EP055, a potential non-hormonal male contraceptive

Michael G. O’Rand, Katherine G. Hamil, Tiffany Adevai, Mary Zelinski

PLOS One April 19 2018 

also check out the Male Contraceptive Initiative Website

Male Contraception Initiative works with researchers across the globe to make male contraceptive options a reality.